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polyvinylidene fluoride membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad polyvinylidene fluoride membrane
    Polyvinylidene Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+fluoride+pvdf+membranes/Trans-Blot+Turbo+RTA+Midi+PVDF+Transfer+Kit/pm42126728-109-18-21
    Average 96 stars, based on 194 article reviews
    polyvinylidene fluoride membrane - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Cell type-specific control of cardiometabolic disease by the dileucine motif of the LDL receptor-related protein LRP1
    Article Snippet: .. The plasma samples were also subjected to SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride (PVDF) membranes (162-0177, BioRad) for Western blot analysis with antibodies against apoB, apoE, and apoA-I. ..

    Nucleic Acid Electrophoresis:

    Article Title: Cell type-specific control of cardiometabolic disease by the dileucine motif of the LDL receptor-related protein LRP1
    Article Snippet: .. The plasma samples were also subjected to SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride (PVDF) membranes (162-0177, BioRad) for Western blot analysis with antibodies against apoB, apoE, and apoA-I. ..

    Article Title: Lactylome Reprogramming Mediates Therapeutic Response and Adaptation to Neoadjuvant Chemotherapy in Esophageal Squamous Cell Carcinoma.
    Article Snippet: .. Equal amounts of protein were denatured at 95°C for 5 min, resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene fluoride (PVDF) membranes (1620177, Bio-Rad). ..

    Article Title: Grape seed proanthocyanidin extract suppresses bladder cancer by dual blockade of IMPDH1/2-mediated purine and pyrimidine nucleotide biosynthesis.
    Article Snippet: Ethnopharmacological relevance: Grape seed proanthocyanidin extract (GSPE), derived from Vitis vinifera L., has been used in traditional European and Mediterranean medicine to treat inflammation, vascular diseases, and wounds.. Historically employed for “detoxification” and “hard lumps” (possibly tumors), its effects are now attributed to GSPE's antioxidant and antiproliferative properties.. Modern studies suggest these mechanisms may underlie its potential anticancer applications, including against bladder cancer.

    Western Blot:

    Article Title: Cell type-specific control of cardiometabolic disease by the dileucine motif of the LDL receptor-related protein LRP1
    Article Snippet: .. The plasma samples were also subjected to SDS-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride (PVDF) membranes (162-0177, BioRad) for Western blot analysis with antibodies against apoB, apoE, and apoA-I. ..

    Incubation:

    Article Title: Generation of an autophagy-targeting influenza A virus as live attenuated vaccine.
    Article Snippet: .. Then the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad, Cat# 1620177), followed by incubation AR TI CL E IN P RE SS with 5% skim milk (YEASEN, Cat# 36120ES76) in TBST (EpiZyme Solarbio, Cat# PS103 T1081) for 1 h at room temperature. ..

    Article Title: ADAM10‐Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell‐Derived Small Extracellular Vesicles
    Article Snippet: .. Proteins were transferred onto activated polyvinylidene fluoride (PVDF) membranes (BioRad, Cat# 1620177) in blotting buffer (192 mM glycine, 0.1% (w/v) SDS, 15% (v/v) methanol, 25 mM Tris‐base, pH 8.3) using a wet blot system at a constant voltage of 110 V for 1 h. Membranes were blocked with 5% milk powder in Tris‐buffered saline containing Tween‐20 (20 mM Tris pH 7.4, 150 mM NaCl, 0.1% Tween‐20) (TBS‐T) for 1 h at RT and incubated overnight at 4°C with primary antibodies against ADAM10 (polyclonal, C‐terminal, 1:1000, Abcam, Cat# ab1997; clone EPR5622, C‐terminal, 1:1000, Abcam, Cat# ab124695), ALIX (clone E6P9B, 1:1000, Cell Signalling, Cat# 92880), flotillin‐1 (clone 18/Flotillin‐1, 1:1000, BD Biosciences, Cat# 610820), GM130 (clone 35/GM130 (RUO), 1:500, BD Biosciences, Cat# 610823), LC3B (polyclonal, 1:1000, Abcam, Cat# ab48394), APP and APP‐CTFs (clone Y188, 1:1000, Abcam, Cat# ab32136), LRP1 (clone 11H4, 1:1000, BioLegend, Cat# 858701), or NCAM1 (polyclonal, 1:1000, R&D, Cat# AF2408). .. Corresponding secondary antibodies conjugated with horseradish peroxidase (HRP) (anti‐mouse and anti‐rabbit, 1:10,000, Jackson ImmunoResearch; anti‐goat, 1:25,000, Sigma, Cat# A5420) were applied for 1 h at RT in TBS‐T containing 5% milk powder.

    Saline:

    Article Title: ADAM10‐Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell‐Derived Small Extracellular Vesicles
    Article Snippet: .. Proteins were transferred onto activated polyvinylidene fluoride (PVDF) membranes (BioRad, Cat# 1620177) in blotting buffer (192 mM glycine, 0.1% (w/v) SDS, 15% (v/v) methanol, 25 mM Tris‐base, pH 8.3) using a wet blot system at a constant voltage of 110 V for 1 h. Membranes were blocked with 5% milk powder in Tris‐buffered saline containing Tween‐20 (20 mM Tris pH 7.4, 150 mM NaCl, 0.1% Tween‐20) (TBS‐T) for 1 h at RT and incubated overnight at 4°C with primary antibodies against ADAM10 (polyclonal, C‐terminal, 1:1000, Abcam, Cat# ab1997; clone EPR5622, C‐terminal, 1:1000, Abcam, Cat# ab124695), ALIX (clone E6P9B, 1:1000, Cell Signalling, Cat# 92880), flotillin‐1 (clone 18/Flotillin‐1, 1:1000, BD Biosciences, Cat# 610820), GM130 (clone 35/GM130 (RUO), 1:500, BD Biosciences, Cat# 610823), LC3B (polyclonal, 1:1000, Abcam, Cat# ab48394), APP and APP‐CTFs (clone Y188, 1:1000, Abcam, Cat# ab32136), LRP1 (clone 11H4, 1:1000, BioLegend, Cat# 858701), or NCAM1 (polyclonal, 1:1000, R&D, Cat# AF2408). .. Corresponding secondary antibodies conjugated with horseradish peroxidase (HRP) (anti‐mouse and anti‐rabbit, 1:10,000, Jackson ImmunoResearch; anti‐goat, 1:25,000, Sigma, Cat# A5420) were applied for 1 h at RT in TBS‐T containing 5% milk powder.



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